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KMID : 0364219910340010001
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Korean Journal of Zoology
1991 Volume.34 No. 1 p.1 ~ p.7
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Isolation and Characterization of Lipophorin from Lymantria dispar L.
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Ryu Jae-Ku
Kim Hak-Ryul
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Abstract
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Lipophorin of Lymuntrn’adispor L. has been purified by KBr density gradient ultracentrifugation. The properties and synthetic site of lipophorin and quantitative change of lipophorin during development have been determined using electrophoresis and immunological analysis. Lipophorin is composed of ho subunits. apo-Lpl (230.000), ago-Lpll (49,000). ann contains carbohydrates and lipids. Anti-lipophorin showed positive reactions with fat body extract and ovary extract but not with gut extract. The concentration of lipophorin in hemolymph showed gradual decrease during larval and pupal stages. Also. fat body released lipophorin into medium. Immunological test showed some partial identity between lipophorin of Lymantpia dispar and hemolymph proteins (probably lipophorin) of Hyphontria cuneo and Galleria metlonefla.
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KEYWORD
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Hemolymph, Fat body, Lipohorin, Immunological analysis, Lymantria dispar
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